Dog ELA2(Elastase 2, Neutrophil) ELISA Kit

Mfr.No.

ELK5911

Alternative Names

ELANE; HLE; HNE; NE; PMN-E; Neutrophil Elastase; Medullasin; Polymorphonuclear Leukocyte Elastase; Bone Marrow Serine Protease; Human Leukocyte Elastase; PMN Elastase

Reactivity

Dog

Detection Range

31.25-2000 pg/mL

Sample Type

Other biological fluids, Serum, Plasma

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant ELA2 and the recovery rates were calculated by comparing the measured value to the expected amount of ELA2 in samples.

Matrix	Recovery range	Average
serum(n=5)	78-90%	84%
EDTA plasma(n=5)	89-107%	98%
Heparin plasma(n=5)	87-99%	93%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ELA2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-92%	83-96%	93-102%	87-98%
EDTA plasma(n=5)	89-102%	92-101%	88-102%	87-102%
Heparin plasma(n=5)	93-104%	83-96%	95-103%	88-95%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
2000.00	1.908	1.809
1000.00	1.511	1.412
500.00	1.073	0.974
250.00	0.872	0.773
125.00	0.521	0.422
62.50	0.361	0.262
31.25	0.186	0.087
0.00	0.099	0.000

Sensitivity

13.5 pg/mL

Limit Of Detection

2000 pg/mL

Antigen	ELA2
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog ELA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog ELA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog ELA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog ELA2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Enzyme & Kinase;Infection immunity;
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE15207441	ELK5911	48T	$412		Add to Cart
BHE15207441	ELK5911	96T	$588		Add to Cart

Dog ELA2(Elastase 2, Neutrophil) ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Dog ELA2(Elastase 2, Neutrophil) ELISA Kit

Rate it now..!!