Dog cTnT/TNNT2(Troponin T Type 2, Cardiac) ELISA Kit

Mfr.No.

ELK8127

Sensitivity

23.9 pg/mL

Detection Range

62.5-4000 pg/mL

Reactivity

Dog

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

CMH2; cTnT; TnTc; Cardiomyopathy,Hypertrophic 2; Cardiac muscle troponin T; TNNT2; Troponin T Type 2, Cardiac

Recovery

Matrices listed below were spiked with certain level of recombinant cTnT/TNNT2 and the recovery rates were calculated by comparing the measured value to the expected amount of cTnT/TNNT2 in samples.

Matrix	Recovery range	Average
serum(n=5)	88-101%	94%
EDTA plasma(n=5)	82-95%	88%
Heparin plasma(n=5)	78-91%	84%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of cTnT/TNNT2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	87-102%	85-94%	87-101%	89-99%
EDTA plasma(n=5)	86-101%	86-101%	87-96%	92-105%
Heparin plasma(n=5)	89-102%	92-101%	88-102%	87-102%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
4000.00	2.268	2.182
2000.00	1.562	1.476
1000.00	1.067	0.981
500.00	0.872	0.786
250.00	0.529	0.443
125.00	0.385	0.299
62.50	0.206	0.120
0.00	0.086	0.000

Limit Of Detection

4000 pg/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog cTnT/TNNT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog cTnT/TNNT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog cTnT/TNNT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog cTnT/TNNT2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

Applications	ELISA
Research Area	Cardiovascular biology;

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE15207402	ELK8127	48T	$412		Add to Cart
BHE15207402	ELK8127	96T	$588		Add to Cart

Dog cTnT/TNNT2(Troponin T Type 2, Cardiac) ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Dog cTnT/TNNT2(Troponin T Type 2, Cardiac) ELISA Kit

Rate it now..!!