Dog Complement 3(C3)ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL003888DO

Sensitivity

0.195 ug/mL

Detection Range

0.78 ug/mL-50 ug/mL

Reactivity

Canis familiaris, Dog

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Complement 3(C3), AHUS5, ARMD9, ASP, CPAMD1, acylation-stimulating protein cleavage product, complement component C3

Recovery

The recovery of mouse SEMA3A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	93-96
EDTA plasma (n=4)	100	95-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SEMA3A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	88
	Range %	84-92
1:200	Average %	94
	Range %	90-98
1:400	Average %	99
	Range %	95-104
1:800	Average %	102
	Range %	96-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Canis lupus familiaris

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	C3

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10500550	CSB-EL003888DO	10x96T	$5652.50	Add to Cart