Dog clusterin,CLU ELISA Kit

Dog clusterin,CLU ELISA Kit

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Mfr.No.

CSB-E13770c

Sensitivity

4.963 ng/mL

Detection Range

7.813 ng/mL-500 ng/mL

Reactivity

Canis familiaris, Dog

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

clusterin, AAG4, APOJ, CLI, KUB1, MGC24903, SGP-2, SGP2, SP-40, TRPM-2, TRPM2, aging-associated protein 4, apolipoprotein J, clusterin (complement lysis inhibitor, SP-40,40, sulfated glycoprotein 2, testosterone

Recovery

The recovery of mouse BMP-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	95-105
EDTA plasma (n=4)	102	96-108

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse BMP-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-102
1:2	Average %	96
	Range %	82-101
1:4	Average %	98
	Range %	93-107
1:8	Average %	97
	Range %	92-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.616	2.582	2.599	2.414
1000	2.215	2.234	2.225	2.040
500	1.801	1.834	1.818	1.633
250	1.367	1.330	1.349	1.164
125	0.768	0.743	0.756	0.571
62.5	0.469	0.442	0.456	0.271
31.25	0.272	0.292	0.282	0.097
0	0.184	0.186	0.185	?

Species

Canis lupus familiaris

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P25473

Applications	ELISA
Research Area	CLU

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10500548	CSB-E13770c	96T	$495	Add to Cart
BHE10500548	CSB-E13770c	5x96T	$2376	Add to Cart
BHE10500548	CSB-E13770c	10x96T	$4702.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.