Matrices listed below were spiked with certain level of DHEA-S and the recovery rates were calculated by comparing the measured value to the expected amount of DHEA-S in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
95-104
98
EDTA plasma(n=5)
96-101
99
heparin plasma(n=5)
96-104
100
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DHEA-S and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
91-99%
92-105%
90-104%
EDTA plasma(n=5)
93-99%
89-101%
91-100%
heparin plasma(n=5)
91-100%
90-98%
93-98%
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Notes
For Research Use Only
Applications
ELISA
Applications Notes
DHEA-S ELISA Kit allows for the in vitro quantitative determination of DHEA-S concentrations in serum, plasma, tissue homogenates and other biological fluids.
Storage
2-8 °C for 6 months
Reviews of DHEA-S (Dehydroepiandrosterone Sulfate) ELISA Kit