Mouse Interleukin 1 Beta (IL1b) (CLIA) ELISA Kit

Mfr.No.

SCA563Mu

Description

This assay has high sensitivity and excellent specificity for detection of Interleukin 1 Beta (IL1b).
No significant cross-reactivity or interference between Interleukin 1 Beta (IL1b) and analogues was observed.

Alternative Names

IL1-B; IL1-Beta; IL1F2; IL-1?; Interleukin-1 Family Member 2; Catabolin

Reactivity

Mouse

Detection Method

Chemiluminescence

Detection Range

1.37-1000pg/mL

Sample Type

Tissue homogenates, Cell lysates, Cell culture supernates, Other biological fluids, Serum, Plasma

Assay Type

Sandwich

Assay Duration

2h, 40min

Kit Components

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×120µL	Assay Diluent A	1×12mL
Detection Reagent B	1×120µL	Assay Diluent B	1×12mL
Substrate A	1×10mL	Substrate B	1×2mL
Wash Buffer (30 × concentrate)	1×20mL	Instruction manual	1

Recovery

Matrices listed below were spiked with certain level of recombinant Interleukin 1 Beta (IL1b) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 1 Beta (IL1b) in samples.

Matrix	Recovery range (%)	Average(%)
serum(n=5)	92-103	98
EDTA plasma(n=5)	96-105	101
heparin plasma(n=5)	89-96	93

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 1 Beta (IL1b) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8	1:16
serum(n=5)	88-104%	93-101%	82-98%	79-93%
EDTA plasma(n=5)	80-103%	85-105%	78-102%	84-98%
heparin plasma(n=5)	79-92%	79-104%	84-98%	89-101%

Features

Detects protein (regular version)

Species

Mouse

Sensitivity

0.49pg/mL

Precautions

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Notes

The microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Beta (IL1b). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 1 Beta (IL1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 1 Beta (IL1b) level in the sample or standard.;

Gene ID	16176
UniProt ID	P10749
Antigen	IL1b
Usage Notes	1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C; 8. Read RLU value immediately.

Applications	ELISA
Research Area	Cytokine
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Beta (IL1b) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Beta (IL1b) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: C<10% Inter-Assay: C<12%

Storage	4°C for 1 month/ -20°C for long-term(within shelf life)
Shelf Life	one year

Reference	Poly(ADP-ribose) polymerase 1 inhibition protects cardiomyocytes from inflammation andapoptosis in diabetic cardiomyopathy. Flavonoids from sea buckthorn inhibit the lipopolysaccharide-induced inflammatory response in RAW264.7 macrophages through the MAPK and NF-κB pathways.

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SKU	Supplier No.	Size	Your price
BHE10102129	SCA563Mu	96T*1	$864	Add to Cart
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CLIA Kit for Interleukin 1 Beta (IL1b) (Mouse)

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