Human Growth Factor Receptor Bound Protein 10 (Grb10) (CLIA) ELISA Kit

Mfr.No.

SCF468Hu

Description

This assay has high sensitivity and excellent specificity for detection of Growth Factor Receptor Bound Protein 10 (Grb10).
No significant cross-reactivity or interference between Growth Factor Receptor Bound Protein 10 (Grb10) and analogues was observed.

Alternative Names

GRB-IR; IRBP; MEG1; RSS; GRB10 adapter protein; Insulin receptor-binding protein Grb-IR

Reactivity

Human

Detection Method

Chemiluminescence

Detection Range

27.4-20,000pg/mL

Sample Type

Tissue homogenates, Other biological fluids, Serum, Plasma

Assay Type

Sandwich

Assay Duration

2h, 40min

Kit Components

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×120µL	Assay Diluent A	1×12mL
Detection Reagent B	1×120µL	Assay Diluent B	1×12mL
Substrate A	1×10mL	Substrate B	1×2mL
Wash Buffer (30 × concentrate)	1×20mL	Instruction manual	1

Features

Detects protein (regular version)

Species

Human

Sensitivity

10.2pg/mL

Precautions

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Notes

The microplate provided in this kit has been pre-coated with an antibody specific to Growth Factor Receptor Bound Protein 10 (Grb10). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Growth Factor Receptor Bound Protein 10 (Grb10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Growth Factor Receptor Bound Protein 10 (Grb10) level in the sample or standard.;

Gene ID	2887
UniProt ID	Q13322
Antigen	Grb10
Usage Notes	1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C; 8. Read RLU value immediately.

Applications	ELISA
Research Area	Cytokine
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Factor Receptor Bound Protein 10 (Grb10) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Factor Receptor Bound Protein 10 (Grb10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: C<10% Inter-Assay: C<12%

Storage	4°C for 1 month/ -20°C for long-term(within shelf life)
Shelf Life	one year

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price
BHE10106938	SCF468Hu	96T*1	$840	Add to Cart
BHE10106938	SCF468Hu	96T*5	$3780	Add to Cart
BHE10106938	SCF468Hu	96T*10	$7140	Add to Cart

CLIA Kit for Growth Factor Receptor Bound Protein 10 (Grb10) (Human)

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of CLIA Kit for Growth Factor Receptor Bound Protein 10 (Grb10) (Human)

Rate it now..!!