Rat Growth Differentiation Factor 11 (GDF11) (CLIA) ELISA Kit

Mfr.No.

SCC113Ra

Sensitivity

0.53pg/mL

Detection Range

1.37-1,000pg/mL

Reactivity

Rat

Assay Duration

2h, 40min

Assay Type

Sandwich

Description

This assay has high sensitivity and excellent specificity for detection of Growth Differentiation Factor 11 (GDF11).
No significant cross-reactivity or interference between Growth Differentiation Factor 11 (GDF11) and analogues was observed.

Alternative Names

BMP11; Bone morphogenetic protein 11

Detection Method

Chemiluminescence

Kit Components

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×120µL	Assay Diluent A	1×12mL
Detection Reagent B	1×120µL	Assay Diluent B	1×12mL
Substrate A	1×10mL	Substrate B	1×2mL
Wash Buffer (30 × concentrate)	1×20mL	Instruction manual	1

Recovery

Matrices listed below were spiked with certain level of recombinant Growth Differentiation Factor 11 (GDF11) and the recovery rates were calculated by comparing the measured value to the expected amount of Growth Differentiation Factor 11 (GDF11) in samples.

Matrix	Recovery range (%)	Average(%)
serum(n=5)	78-105	101
EDTA plasma(n=5)	95-104	101
heparin plasma(n=5)	92-102	99

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Growth Differentiation Factor 11 (GDF11) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8	1:16
serum(n=5)	94-101%	97-104%	80-104%	79-94%
EDTA plasma(n=5)	82-99%	93-103%	83-102%	96-103%
heparin plasma(n=5)	79-94%	90-101%	81-89%	91-98%

Features

Detects protein (regular version)

Species

Rat

Sample Type

Cell lysates, Tissue homogenates, Other biological fluids

Precautions

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Differentiation Factor 11 (GDF11) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Differentiation Factor 11 (GDF11) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: C<10%
Inter-Assay: C<12%

Notes

The microplate provided in this kit has been pre-coated with an antibody specific to Growth Differentiation Factor 11 (GDF11). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Growth Differentiation Factor 11 (GDF11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Growth Differentiation Factor 11 (GDF11) level in the sample or standard.;

Usage Notes

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Protocol

Datasheet

Gene ID	29454
UniProt ID	Q9Z217

Applications	ELISA
Research Area	Cytokine

Storage	4°C for 1 month/ -20°C for long-term(within shelf life)
Shelf Life	one year

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SKU	Supplier No.	Size	Your price
BHE10105103	SCC113Ra	96T*1	$912	Add to Cart
BHE10105103	SCC113Ra	96T*5	$4104	Add to Cart
BHE10105103	SCC113Ra	96T*10	$7752	Add to Cart

CLIA Kit for Growth Differentiation Factor 11 (GDF11) (Rat)

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