Human Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) (CLIA) ELISA Kit

Mfr.No.

SCB246Hu

Description

This assay has high sensitivity and excellent specificity for detection of Cytokeratin Fragment Antigen 21-1 (CYFRA21-1).
No significant cross-reactivity or interference between Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) and analogues was observed.

Reactivity

Human

Detection Method

Chemiluminescence

Detection Range

27.4-20,000pg/mL

Sample Type

Tissue homogenates, Cell lysates, Other biological fluids

Assay Type

Sandwich

Assay Duration

2h, 40min

Kit Components

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×120µL	Assay Diluent A	1×12mL
Detection Reagent B	1×120µL	Assay Diluent B	1×12mL
Substrate A	1×10mL	Substrate B	1×2mL
Wash Buffer (30 × concentrate)	1×20mL	Instruction manual	1

Recovery

Matrices listed below were spiked with certain level of recombinant Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) and the recovery rates were calculated by comparing the measured value to the expected amount of Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) in samples.

Matrix	Recovery range (%)	Average(%)
serum(n=5)	97-105	101
EDTA plasma(n=5)	90-97	93
heparin plasma(n=5)	80-90	84

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8	1:16
serum(n=5)	97-105%	93-101%	90-98%	97-105%
EDTA plasma(n=5)	80-88%	92-101%	94-102%	86-94%
heparin plasma(n=5)	80-95%	90-98%	89-104%	80-96%

Features

Detects protein (regular version)

Species

Human

Sensitivity

9.9pg/mL

Precautions

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Notes

The microplate provided in this kit has been pre-coated with an antibody specific to Cytokeratin Fragment Antigen 21-1 (CYFRA21-1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Cytokeratin Fragment Antigen 21-1 (CYFRA21-1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) level in the sample or standard.;

Gene ID	3880
UniProt ID	P08727
Antigen	CYFRA21-1
Usage Notes	1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C; 8. Read RLU value immediately.

Applications	ELISA
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: C<10% Inter-Assay: C<12%

Storage	4°C for 1 month/ -20°C for long-term(within shelf life)
Shelf Life	one year

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SKU	Supplier No.	Size	Your price
BHE10103669	SCB246Hu	96T*1	$840	Add to Cart
BHE10103669	SCB246Hu	96T*5	$3780	Add to Cart
BHE10103669	SCB246Hu	96T*10	$7140	Add to Cart

CLIA Kit for Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) (Human)

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