Human Coagulation Factor VIII (F8) (CLIA) ELISA Kit

Mfr.No.

SCB878Hu

Sensitivity

4.9pg/mL

Detection Range

13.7-10,000pg/mL

Reactivity

Human

Assay Duration

2h, 40min

Assay Type

Sandwich

Description

This assay has high sensitivity and excellent specificity for detection of Coagulation Factor VIII (F8).
No significant cross-reactivity or interference between Coagulation Factor VIII (F8) and analogues was observed.

Alternative Names

FVIII; AHF; HEMA; F8B; F8C; Antihemophilic Globulin; Hemophilia A; Anti-Hemophilic Factor A; FVIII Procoagulant Component; F8 Protein

Detection Method

Chemiluminescence

Kit Components

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×120µL	Assay Diluent A	1×12mL
Detection Reagent B	1×120µL	Assay Diluent B	1×12mL
Substrate A	1×10mL	Substrate B	1×2mL
Wash Buffer (30 × concentrate)	1×20mL	Instruction manual	1

Recovery

Matrices listed below were spiked with certain level of recombinant Coagulation Factor VIII (F8) and the recovery rates were calculated by comparing the measured value to the expected amount of Coagulation Factor VIII (F8) in samples.

Matrix	Recovery range (%)	Average(%)
sodium citrate plasma(n=5)	79-101	96

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Coagulation Factor VIII (F8) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8	1:16
sodium citrate plasma(n=5)	81-104%	78-89%	95-105%	96-103%

Features

Detects protein (regular version)

Species

Human

Precautions

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coagulation Factor VIII (F8) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coagulation Factor VIII (F8) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: C<10%
Inter-Assay: C<12%

Notes

The microplate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor VIII (F8). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Coagulation Factor VIII (F8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Coagulation Factor VIII (F8) level in the sample or standard.;

Usage Notes

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

UniProt ID	P00741

Applications	ELISA
Research Area	Metabolic pathway

Storage	4°C for 1 month/ -20°C for long-term(within shelf life)
Shelf Life	one year

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SKU	Supplier No.	Size	Your price
BHE10109379	SCB878Hu	96T*1	$798	Add to Cart
BHE10109379	SCB878Hu	96T*5	$3591	Add to Cart
BHE10109379	SCB878Hu	96T*10	$6783	Add to Cart

CLIA Kit for Coagulation Factor VIII (F8) (Human)

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