General species Arginine (Arg) (Competitive CLIA) ELISA Kit

Mfr.No.

CCB938Ge

Sensitivity

146.9ng/mL

Detection Range

390.6-100,000ng/mL

Reactivity

General

Assay Duration

Assay Type

Competitive Inhibition

Description

This assay has high sensitivity and excellent specificity for detection of Arginine (Arg).
No significant cross-reactivity or interference between Arginine (Arg) and analogues was observed.

Alternative Names

(S)-2-Amino-5-Guanidinopentanoic Acid

Detection Method

Chemiluminescence

Kit Components

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×120µL	Assay Diluent A	1×12mL
Detection Reagent B	1×120µL	Assay Diluent B	1×12mL
Substrate A	1×10mL	Substrate B	1×2mL
Wash Buffer (30 × concentrate)	1×20mL	Instruction manual	1

Recovery

Matrices listed below were spiked with certain level of Arginine (Arg) and the recovery rates were calculated by comparing the measured value to the expected amount of Arginine (Arg) in samples.

Matrix	Recovery range (%)	Average(%)
serum(n=5)	90-104	96
EDTA plasma(n=5)	98-105	102
heparin plasma(n=5)	88-102	95

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Arginine (Arg) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8	1:16
serum(n=5)	88-98%	99-105%	78-99%	90-102%
EDTA plasma(n=5)	94-101%	79-103%	79-90%	79-94%
heparin plasma(n=5)	83-91%	80-88%	81-95%	98-105%

Features

Detects small molecule

Species

General species

Precautions

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arginine (Arg) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arginine (Arg) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: C<10%
Inter-Assay: C<12%

Notes

The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Arginine (Arg). A competitive inhibition reaction is launched between biotin labeled Arginine (Arg) and unlabeled Arginine (Arg) (Standards or samples) with the pre-coated antibody specific to Arginine (Arg). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Arginine (Arg) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Arginine (Arg) level in the sample or standard.

Usage Notes

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
7. Read RLU value immediately.

Protocol

Datasheet

Applications	ELISA
Research Area	Metabolic pathway

Storage	4°C for 1 month/ -20°C for long-term(within shelf life)
Shelf Life	one year

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SKU	Supplier No.	Size	Your price
BHE10104709	CCB938Ge	96T*1	$816	Add to Cart
BHE10104709	CCB938Ge	96T*5	$3672	Add to Cart
BHE10104709	CCB938Ge	96T*10	$6936	Add to Cart

CLIA Kit for Arginine (Arg) (General species)

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