Chicken Tri-iodothyronine,T3 ELISA Kit

Chicken Tri-iodothyronine,T3 ELISA Kit

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Mfr.No.

CSB-E13270C

Alternative Names

Tri-iodothyronine,T3

Reactivity

Chicken

Detection Range

0.5 ng/mL-8 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human ADM2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	97-107
EDTA plasma (n=4)	92	89-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ADM2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	87-99
1:2	Average %	98
	Range %	93-103
1:4	Average %	84
	Range %	81-89
1:8	Average %	89
	Range %	83-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.988	3.036	3.012	2.874
1000	2.365	2.488	2.427	2.289
500	1.609	1.675	1.642	1.504
250	0.901	0.909	0.905	0.767
125	0.545	0.525	0.535	0.397
62.5	0.375	0.389	0.382	0.244
31.25	0.278	0.258	0.268	0.130
0	0.135	0.140	0.138

Species

Gallus gallus

Sensitivity

0.5 ng/mL

Limit Of Detection

450 nm

Antigen	Tri-iodothyronine,T3

Applications

ELISA

Research Area

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Association of heat shock protein 70 gene polymorphisms with acute thermal tolerance, growth, and egg production traits of native chickens in Taiwan Liang H M.et al,Arch. Anim. Breed.,,/ Growth and male reproduction improving of non-thermal dielectric barrier discharge plasma treatment on chickens Jiao Jiao Zhang.et al,J PHYS D APPL PHYS,April 12 Thermal manipulation during broiler chicken embryogenesis increases basal mRNA levels and alters production dynamics of heat shock proteins 70 and 60 and heat shock factors 3 and 4 during thermal stress M B Al-Zghoul.et al,Poultry Science,June 15

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SKU	Supplier No.	Size	Your price
BHE10500505	CSB-E13270C	96T	$495	Add to Cart
BHE10500505	CSB-E13270C	5x96T	$2376	Add to Cart
BHE10500505	CSB-E13270C	10x96T	$4702.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.