Chicken Tri-iodothyronine,T3 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E13270C

Sensitivity

0.5 ng/mL

Detection Range

0.5 ng/mL-8 ng/mL

Reactivity

Chicken

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Tri-iodothyronine,T3

Recovery

The recovery of human ADM2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	97-107
EDTA plasma (n=4)	92	89-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ADM2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	87-99
1:2	Average %	98
	Range %	93-103
1:4	Average %	84
	Range %	81-89
1:8	Average %	89
	Range %	83-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Gallus gallus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	T3

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Association of heat shock protein 70 gene polymorphisms with acute thermal tolerance, growth, and egg production traits of native chickens in Taiwan Liang H M.et al,Arch. Anim. Breed.,,/ Growth and male reproduction improving of non-thermal dielectric barrier discharge plasma treatment on chickens Jiao Jiao Zhang.et al,J PHYS D APPL PHYS,April 12 Thermal manipulation during broiler chicken embryogenesis increases basal mRNA levels and alters production dynamics of heat shock proteins 70 and 60 and heat shock factors 3 and 4 during thermal stress M B Al-Zghoul.et al,Poultry Science,June 15

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