Chicken thyroxine (T4) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E15787C

Alternative Names

thyroxine (T4)

Reactivity

Chicken

Detection Range

20 ng/mL-320 ng/mL

Sample Type

Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human VEGF-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-105
EDTA plasma (n=4)	96	92-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human VEGF-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	88
	Range %	80-96
1:2	Average %	100
	Range %	95-104
1:4	Average %	99
	Range %	92-110
1:8	Average %	89
	Range %	86-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Gallus gallus

Sensitivity

20 ng/mL

Limit Of Detection

450 nm

Antigen	Thyroxine (T4)

Applications

ELISA

Research Area

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Growth and male reproduction improving of non-thermal dielectric barrier discharge plasma treatment on chickens Jiao Jiao Zhang.et al,J PHYS D APPL PHYS,April 12 Thermal manipulation during broiler chicken embryogenesis increases basal mRNA levels and alters production dynamics of heat shock proteins 70 and 60 and heat shock factors 3 and 4 during thermal stress M B Al-Zghoul.et al,Poultry Science,June 15

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BHE10500501	CSB-E15787C	96T	$495	Add to Cart
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BHE10500501	CSB-E15787C	10x96T	$4702.50	Add to Cart