Matrices listed below were spiked with certain level of SPCA/HSP90AA1 and the recovery rates were calculated by comparing the measured value to the expected amount of SPCA/HSP90AA1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-104
96
EDTA plasma(n=5)
85-104
94
heparin plasma(n=5)
85-102
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SPCA/HSP90AA1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-101%
88-102%
92-101%
EDTA plasma(n=5)
90-100%
83-101%
82-100%
heparin plasma(n=5)
82-95%
81-97%
86-96%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
P11501
Applications
ELISA
Applications Notes
SPCAELISA Kit allows for the in vitro quantitative determination of SPCA/HSP90AA1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Chicken SPCA(Heat shock protein HSP 90-alpha)ELISA Kit