Chicken Proliferating Cell Nuclear Antigen

Mfr.No.

E0260Ch

Alternative Names

PCNA;Proliferating cell nuclear antigen

Reactivity

Chicken

Detection Range

1-400ng/ml

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

28888

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.48ng/ml

Background

This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.

Gene ID	373984
UniProt ID	Q9DEA3
Antigen	PCNA

Research Area

Others

Protocol

These standard curves of E0260Ch are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240ng/ml	2.281	2.213	2.167
	2.146
120ng/ml	1.842	1.9	1.854
	1.959
60ng/ml	1.092	1.183	1.137
	1.274
30ng/ml	0.692	0.721	0.675
	0.751
15ng/ml	0.363	0.389	0.343
	0.416
0ng/ml	0.061	0.046	0
	0.032

Precision

We measured random samples of E0260Ch within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	76.37	4.11	5.38
Intra-Assay	2	18	14.4	0.74	5.14
Intra-Assay	3	18	33.94	1.72	5.07

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101166	E0260Ch	48T	$360		Add to Cart
BHE12101166	E0260Ch	96T	$498		Add to Cart

Chicken Proliferating Cell Nuclear Antigen - PCNA ELISA Kit

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