Matrices listed below were spiked with certain level of POSTN/OSF2 and the recovery rates were calculated by comparing the measured value to the expected amount of POSTN/OSF2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-96
92
EDTA plasma(n=5)
85-105
94
heparin plasma(n=5)
88-101
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of POSTN/OSF2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-105%
87-101%
85-99%
EDTA plasma(n=5)
85-101%
88-101%
84-99%
heparin plasma(n=5)
81-95%
80-100%
83-99%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
A0A3Q2U321
Applications
ELISA
Applications Notes
POSTN/OSF2 ELISA Kit allows for the in vitro quantitative determination of POSTN/OSF2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Chicken POSTN/OSF2(Periostin) ELISA Kit