Matrices listed below were spiked with certain level of Pg and the recovery rates were calculated by comparing the measured value to the expected amount of Pg in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-103
94
EDTA plasma(n=5)
86-103
95
heparin plasma(n=5)
92-103
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pg and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.