Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of NPY and the recovery rates were calculated by comparing the measured value to the expected amount of NPY in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-104
97
EDTA plasma(n=5)
88-103
98
heparin plasma(n=5)
93-105
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NPY and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-101%
90-104%
86-103%
EDTA plasma(n=5)
87-99%
82-97%
85-97%
heparin plasma(n=5)
82-98%
82-100%
83-88%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
P28673
Applications
ELISA
Applications Notes
NPYELISA Kit allows for the in vitro quantitative determination of NPY concentrations in serum, plasma, tissue homogenates and other biological fluids.