Chicken Metallothionein

Mfr.No.

E0017Ch

Sensitivity

0.06ng/ml

Detection Range

0.1-40ng/ml

Reactivity

Chicken

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Chicken Metallothionein (also known as MT) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Metallothionein;MT;MT2A;MT4L

Molecular Weight

6462

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0017Ch within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	4.2	0.22	5.21
Intra-Assay	2	18	6.73	0.25	3.7
Intra-Assay	3	18	1.83	0.08	4.33

Background

Metallothioneins have a high content of cysteine residues that bind various heavy metals.

Protocol

These standard curves of E0017Ch are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	2.297	2.19	2.149
	2.083
12ng/ml	1.915	1.914	1.873
	1.913
6ng/ml	1.262	1.223	1.182
	1.184
3ng/ml	0.717	0.698	0.657
	0.678
1.5ng/ml	0.384	0.41	0.369
	0.436
0ng/ml	0.05	0.041	0
	0.032

Gene ID	396212
UniProt ID	P68497

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100969	E0017Ch	48T	$360		Add to Cart
BHE12100969	E0017Ch	96T	$498		Add to Cart

Chicken Metallothionein - MT ELISA Kit

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