Chicken Interleukin 6,IL-6 ELISA Kit

Mfr.No.

CSB-E08549Ch

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Chicken

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

interleukin 6 (interferon, beta 2), BSF2, HGF, HSF, IFNB2, IL-6, B cell stimulatory factor-2, B-cell differentiation factor, CTL differentiation factor, OTTHUMP00000158544, hybridoma growth factor, interleukin 6, interleukin BSF-2

Recovery

The recovery of mouse PYCARD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	86-99
EDTA plasma (n=4)	103	95-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PYCARD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	103
	Range %	95-107
1:2	Average %	95
	Range %	87-99
1:4	Average %	96
	Range %	86-100
1:8	Average %	107
	Range %	96-111

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.168	2.237	2.203	2.042
250	1.522	1.504	1.513	1.352
125	1.000	1.020	1.010	0.849
62.5	0.644	0.682	0.663	0.502
31.25	0.476	0.502	0.489	0.328
15.62	0.390	0.407	0.399	0.238
7.81	0.267	0.259	0.263	0.102
0	0.159	0.163	0.161	?

Species

Gallus gallus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	Q90YI0

Applications	ELISA
Research Area	IL6

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10500469	CSB-E08549Ch	96T	$658	Add to Cart
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BHE10500469	CSB-E08549Ch	10x96T	$6251	Add to Cart

Chicken Interleukin 6,IL-6 ELISA Kit

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