Chicken Interleukin 2,IL-2 ELISA Kit

Chicken Interleukin 2,IL-2 ELISA Kit

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Mfr.No.

CSB-E06755Ch

Alternative Names

Interleukin 2,IL-2

Reactivity

Chicken

Detection Range

0.18 pg/mL-12 pg/mL

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human REN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human REN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	99
	Range %	91-104
1:4	Average %	100
	Range %	92-110
1:8	Average %	95
	Range %	89-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average	Corrected
1000	2.953	2.765	2.859	2.713
500	2.565	2.412	2.489	2.343
250	1.886	1.796	1.841	1.695
125	0.975	0.943	0.959	0.813
62.5	0.501	0.482	0.492	0.346
31.2	0.309	0.301	0.305	0.159
15.6	0.222	0.218	0.220	0.074
0	0.147	0.145	0.146	?

Species

Gallus gallus

Sensitivity

0.04 pg/mL

Limit Of Detection

450 nm

UniProt ID	O73883
Antigen	Interleukin 2,IL-2

Applications

ELISA

Research Area

IL-2

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Lactobacillus acidophilus as a live vehicle for oral immunization against chicken anemia virus Hassan Moeini et al,Tumor Biology,2010 90(1): 77-88 Bursopentine as a Novel Immunoadjuvant Enhances both Humoral and Cell-Mediated Immune Responses to Inactivated H9N2 Avian Influenza Virus in Chickens /,clinical and vaccine immunology,2011 Sep;18(9):1497-502. Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV /,Comparative Immunology, Microbiology and Infectious Diseases,May 34(3):227-36. Improving the potency of DNA vaccine against Chicken Anemia Virus (CAV) by fusing VP1 protein of CAV toMarek's Disease Virus (MDV) Type-1 VP22 protein Hassan Moeini et al,Virology Journal,2011 8:119 Omega-3 polyunsaturated fatty acids enrichment alters performance and immune response in infectious bursal disease challenged broilers Elham Maroufyan et al,Lipids in Health and Disease,2012 11: 15 Consumption of Oxidized Soybean Oil Increased Intestinal Oxidative Stress and Affected Intestinal Immune Variables in Yellow-feathered Broilers Fangfang Liang. et al,Asian Australas. J. Anim. Sci. ,2015 Aug;28(8):1194-201. Eimeria maxima microneme protein 2 delivered as DNA vaccine and recombinant protein induces immunity against experimental homogenous challenge Huang J. et al,Parasitol Int,2015 Oct;64(5):408-16. Immune protection of Microneme 7 (EmMIC7) against Eimeria maxima challenge in chickens Huang J. et al,Avian Pathol,2015 Jul 16:1-35. Immune responses to oral vaccination with Salmonella-delivered avian pathogenic Escherichia coli antigens and protective efficacy against colibacillosis. Lee JH.et al,Can J Vet Res.,2015 79(3):229-34. The molecular characterization and immune protection of microneme 2 of Eimeria acervulina ZC Zhang. et al,Veterinary Parasitology,2015 215: 96-105. Dietary Supplementation of Phoenix dactylifera Seeds Enhances Performance, Immune Response, and Antioxidant Status in Broilers Ali H. El-Far.et al,Oxidative Medicine and Cellular Longevity,/ Immunization with avian metapneumovirus harboring chicken Fc induces higher immune responses. Paudel S.et al,Virus Res.,220:129-35 The Molecular Characterization and Immunity Identification of Microneme 3 of Eimeria acervulina. Zhang Z.et al,J Eukaryot Microbiol.,/ The effect of heat stress on intestinal integrity and Salmonella invasion in broiler birds Alhenaky A.et al,J Therm Biol.,Dec Effects of modified montmorillonite adsorbent on performance, egg quality, serum biochemistry, oxidation status, and immune response of laying hens in late Qu, X. Y.et al,Livestock Science,Jan The molecular characterization and protective efficacy of microneme 3 of Eimeria mitis in chickens XinmeiHuang.et al,Veterinary Parasitology,June 25 Dietary Supplementation of Purified Amino Acid Derived from Animal Blood on Immune Response and Growth Performance of Broiler Chicken T. G. Wandita.et al,Tropical Animal Science Journal,March 5 Effects of three probiotic Bacillus on growth performance, digestive enzyme activities, antioxidative capacity, serum immunity, and biochemical parameters in broilers Li Gong.et al,animal science journal,September 9

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.