Chicken Interleukin 1beta,IL-1beta ELISA Kit

Chicken Interleukin 1beta,IL-1beta ELISA Kit

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Mfr.No.

CSB-E11230Ch

Alternative Names

Interleukin 1beta,IL-1beta, IL-1beta,Interleukin 1beta

Reactivity

Chicken

Detection Range

0.27 pg/mL-200 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human PYCARD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PYCARD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-102
1:2	Average %	90
	Range %	85-99
1:4	Average %	98
	Range %	92-106
1:8	Average %	92
	Range %	86-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	1.834	1.867	1.851	1.713
500	1.517	1.541	1.529	1.391
250	1.213	1.224	1.219	1.081
125	0.892	0.902	0.897	0.759
62.5	0.561	0.578	0.570	0.432
31.2	0.345	0.352	0.349	0.211
15.6	0.256	0.266	0.261	0.123
0	0.137	0.139	0.138	?

Species

Gallus gallus

Sensitivity

0.27 pg/mL

Limit Of Detection

450 nm

UniProt ID	O73909
Antigen	Interleukin 1β,IL-1β

Applications

ELISA

Research Area

IL1B

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	The antimicrobial peptide sublancin ameliorates necrotic enteritis induced by in broilers S Wang. et al,Journal of Animal Science,2015 Oct;93(10):4750-60 Immunization with avian metapneumovirus harboring chicken Fc induces higher immune responses. Paudel S.et al,Virus Res.,220:129-35 Effects of yeast cell wall on growth performance, immune responses and intestinal short chain fatty acid concentrations of broiler in an experimental necrotic enteritis model Guang-DaXue.et al,Animal Nutrition,Aug 18 Dietary L-theanine alleviated lipopolysaccharide-induced immunological stress in yellow-feathered broilers RuiLi.et al,Animal Nutrition,May 23 The protective role of selenium against cadmium-induced hepatotoxicity in laying hens: Expression of Hsps and inflammation-related genes and modulation of elements homeostasis RunxiangZhang.et al,Ecotoxicology and Environmental Safety,May 21 Xylanase and Fermented Polysaccharide of Hericium caputmedusae Reduce Pathogenic Infection of Broilers by Improving Antioxidant and Anti-Inflammatory Properties Sitong Zhang, et al,Oxidative Medicine and Cellular Longevity,December 30

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SKU	Supplier No.	Size	Your price
BHE10500464	CSB-E11230Ch	96T	$658	Add to Cart
BHE10500464	CSB-E11230Ch	5x96T	$3158.40	Add to Cart
BHE10500464	CSB-E11230Ch	10x96T	$6251	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.