Chicken Interleukin 10,IL-10 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E12835C

Alternative Names

interleukin 10, CSIF, IL-10, IL10A, MGC126450, MGC126451, TGIF, cytokine synthesis inhibitory factor

Reactivity

Chicken

Detection Range

1 pg/mL-200 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human GA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	96	92-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human GA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:200	Average %	94
	Range %	90-98
1:400	Average %	94
	Range %	91-98
1:800	Average %	101
	Range %	98-104
1:1600	Average %	88
	Range %	82-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Gallus gallus

Sensitivity

0.5 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q6A2H4
Antigen	Interleukin 10

Applications

ELISA

Research Area

IL10

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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