Chicken Interferon beta,IFN-beta/IFNB ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E11237Ch

Sensitivity

3.125 pg/mL

Detection Range

2.5 pg/mL-200 pg/mL

Reactivity

Chicken

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Interferon beta,IFN-beta/IFNB, IFB-beta,interferon beta

Recovery

The recovery of goat TNF-? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-97
EDTA plasma (n=4)	97	92-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of goat TNF-? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	84
	Range %	81-91
1:2	Average %	98
	Range %	91-103
1:4	Average %	99
	Range %	95-104
1:8	Average %	94
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Gallus gallus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q90873

Applications	ELISA
Research Area	IFN-beta, IFNB

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Differences in expression of genes in the MyD88 and TRIF signalling pathways and methylation of TLR4 and TRIF in Tibetan chickens and DaHeng S03 chickens infected with Salmonella enterica serovar Enteritidis Xiaocheng Li.et al,Veterinary Immunology and Immunopathology,May 11

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