Chicken Inducible Nitric Oxide Synthase

Mfr.No.

E0188Ch

Description

his sandwich kit is for the accurate quantitative detection of Inducible nitric oxide synthase (also known as INOS) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Inducible NO Synthase;Inducible NOS;iNOS;Macrophage NOS;Nitric oxide Synthase, inducible;NOS 2;NOS type II;NOS2;Peptidyl-cysteine S-nitrosylase NOS2;

Reactivity

Chicken

Detection Range

0.3-90ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

129649

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.14ng/ml

Background

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO may serve as both a paracrine and autocrine signal for modulating osteoclast bone resorption. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 (By similarity).

Gene ID	395807
UniProt ID	Q90703
Antigen	NOS2

Research Area

Others

Protocol

These standard curves of E0188Ch are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/ml	2.545	2.478	2.445
	2.411
24ng/ml	1.344	1.396	1.362
	1.447
12ng/ml	0.777	0.813	0.78
	0.85
6ng/ml	0.456	0.424	0.39
	0.391
3ng/ml	0.206	0.233	0.2
	0.261
0ng/ml	0.034	0.033	0
	0.033

Precision

We measured random samples of E0188Ch within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	12.3	0.56	4.5
Intra-Assay	2	18	29.9	0.86	2.9
Intra-Assay	3	18	9.9	0.66	6.7

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101109	E0188Ch	96T	$498		Add to Cart
BHE12101109	E0188Ch	48T	$360		Add to Cart

Chicken Inducible Nitric Oxide Synthase - INOS ELISA Kit

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