Chicken Immunoglobulin of Yolk,IgY ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E11635Ch

Sensitivity

0.02 ng/mL

Detection Range

0.078 ng/mL-5 ng/mL

Reactivity

Chicken

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Immunoglobulin of Yolk,IgY

Recovery

The recovery of mouse ANGPTL2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	101-106
EDTA plasma (n=4)	95	91-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANGPTL2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	91-102
1:2	Average %	102
	Range %	98-106
1:4	Average %	89
	Range %	84-94
1:8	Average %	94
	Range %	92-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Gallus gallus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	IgY

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Zinc Regulates Lipid Metabolism and MMPs Expression in Lipid Disturbance Rabbits Xu C. et al,Biol Trace Elem Res,2015 Dec;168(2):411-20

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