Chicken Immunoglobulin M(IgM)ELISA Kit

Mfr.No.

CSB-E16200C

Sensitivity

7.8 ng/mL

Detection Range

7.8 ng/mL-2000 ng/mL

Reactivity

Chicken

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Immunoglobulin M(IgM)

Recovery

The recovery of human TRAIL-R2/DR5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-103
EDTA plasma (n=4)	86	83-89

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TRAIL-R2/DR5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	84
	Range %	81-89
1:2	Average %	92
	Range %	87-94
1:4	Average %	102
	Range %	98-106
1:8	Average %	105
	Range %	101-109

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Gallus gallus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	IgM

Storage	Store at 2-8°C. Please refer to protocol.

Reference	In Ovo and dietary administration of oligosaccharides extracted from palm kernel cake influence general health of pre- and neonatal broiler chicks Mohammad Faseleh Jahromi.et al,PLoS One,Sep 7 Effects of modified montmorillonite adsorbent on performance, egg quality, serum biochemistry, oxidation status, and immune response of laying hens in late Qu, X. Y.et al,Livestock Science,Jan

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