Chicken Immunoglobulin M(IgM)ELISA Kit

Mfr.No.

CSB-E16200C

Alternative Names

Immunoglobulin M(IgM)

Reactivity

Chicken

Detection Range

7.8 ng/mL-2000 ng/mL

Sample Type

Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human TRAIL-R2/DR5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-103
EDTA plasma (n=4)	86	83-89

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TRAIL-R2/DR5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	84
	Range %	81-89
1:2	Average %	92
	Range %	87-94
1:4	Average %	102
	Range %	98-106
1:8	Average %	105
	Range %	101-109

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.604	2.598	2.601	2.514
1000	2.293	2.204	2.249	2.162
500	1.687	1.658	1.673	1.586
250	1.087	1.002	1.045	0.958
125	0.629	0.638	0.634	0.547
62.5	0.454	0.468	0.461	0.374
31.25	0.247	0.231	0.239	0.152
0	0.085	0.089	0.087	?

Species

Gallus gallus

Sensitivity

7.8 ng/mL

Limit Of Detection

450 nm

Antigen	Immunoglobulin M(IgM)

Applications

ELISA

Research Area

IgM

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

Reference	In Ovo and dietary administration of oligosaccharides extracted from palm kernel cake influence general health of pre- and neonatal broiler chicks Mohammad Faseleh Jahromi.et al,PLoS One,Sep 7 Effects of modified montmorillonite adsorbent on performance, egg quality, serum biochemistry, oxidation status, and immune response of laying hens in late Qu, X. Y.et al,Livestock Science,Jan

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SKU	Supplier No.	Size	Your price
BHE10500449	CSB-E16200C	96T	$595	Add to Cart
BHE10500449	CSB-E16200C	5x96T	$2856	Add to Cart
BHE10500449	CSB-E16200C	10x96T	$5652.50	Add to Cart

Chicken Immunoglobulin M(IgM)ELISA Kit

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