Chicken Heat Shock Protein 90,HSP-90 ELISA Kit

Chicken Heat Shock Protein 90,HSP-90 ELISA Kit

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Mfr.No.

CSB-E12873C

Sensitivity

0.39 ng/mL

Detection Range

1.56 ng/mL-100 ng/mL

Reactivity

Chicken

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

heat shock protein 90kDa alpha (cytosolic), class A member 1, FLJ31884, HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp89, Hsp90, LAP2, heat shock 90kD protein 1, alpha, heat shock 90kD protein 1, alpha-like 4, heat shock 90kD protein, a

Recovery

The recovery of mouse CYR61 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	88-98
EDTA plasma (n=4)	96	92-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CYR61 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	91
	Range %	86-99
1:200	Average %	97
	Range %	92-104
1:400	Average %	93
	Range %	88-98
1:800	Average %	88
	Range %	83-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	2.584	2.652	2.618	2.498
600	2.175	2.075	2.125	2.005
300	1.607	1.603	1.605	1.485
150	1.016	1.090	1.053	0.933
75	0.503	0.524	0.514	0.394
37.5	0.328	0.355	0.342	0.222
18.75	0.232	0.229	0.231	0.111
0	0.120	0.119	0.120	?

Species

Gallus gallus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P11501

Applications	ELISA
Research Area	HSP90AA1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10500446	CSB-E12873C	96T	$790	Add to Cart
BHE10500446	CSB-E12873C	5x96T	$3792	Add to Cart
BHE10500446	CSB-E12873C	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.