Chicken GnRH(Gonadotropin Releasing Hormone) ELISA Kit

Mfr.No.

ELK8495

Alternative Names

GNRH1; GRH; LNRH; LHRH; LH-RH I; Luliberin; Luteinizing-Hormone Releasing Hormone; Progonadoliberin-1; Gonadoliberin I; Gonadorelin; GnRH-associated peptide 1

Reactivity

Chicken

Detection Range

0.25-16 ng/mL

Sample Type

Tissue homogenates, Cell lysates, Other biological fluids, Serum, Plasma, Cell culture supernatants

Assay Type

Competitive Inhibition

Assay Duration

Recovery

Matrices listed below were spiked with certain level of recombinant GnRH and the recovery rates were calculated by comparing the measured value to the expected amount of GnRH in samples.

Matrix	Recovery range	Average
serum(n=5)	82-98%	90%
EDTA plasma(n=5)	80-97%	88%
Heparin plasma(n=5)	82-94%	90%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GnRH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	89-99%	83-96%	85-94%	87-96%
EDTA plasma(n=5)	85-97%	87-96%	85-96%	85-95%
Heparin plasma(n=5)	87-96%	97-105%	79-90%	87-96%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
16.00	0.232
8.00	0.413
4.00	0.611
2.00	0.997
1.00	1.197
0.50	1.578
0.25	1.889
0.00	2.325

Sensitivity

0.1 ng/mL

Limit Of Detection

16 ng/mL

UniProt ID	P37042
Antigen	GnRH
Usage Notes	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken GnRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken GnRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken GnRH in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Endocrinology;Reproductive science;Neuro science;Genetic science;Hormone metabolism;
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15200272	ELK8495	48T	$320		Add to Cart
BHE15200272	ELK8495	96T	$458		Add to Cart

Chicken GnRH(Gonadotropin Releasing Hormone) ELISA Kit

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