Chicken DAO(Diamine Oxidase) ELISA Kit

Mfr.No.

ELK9423

Alternative Names

AOC1, ABP1, KAO, Histaminase, Kidney amine oxidase, Amiloride Binding Protein 1, Amiloride-sensitive amine oxidase, Amine oxidase copper domain-containing protein 1, AOC1; ABP1; KAO; Histaminase; Kidney amine oxidase; Amiloride Binding Protein 1; Amiloride-sensitive amine oxidase; Amine oxidase copper domain-containing protein 1

Reactivity

Chicken

Detection Range

0.63-40 ng/mL, 0.63-40 ng/mL

Sample Type

Tissue homogenates, Cell lysates, Other biological fluids, Serum, Plasma

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant DAO and the recovery rates were calculated by comparing the measured value to the expected amount of DAO in samples.

Matrix	Recovery range	Average
serum(n=5)	81-97%	89%
EDTA plasma(n=5)	80-95%	87%
Heparin plasma(n=5)	86-99%	92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DAO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	83-96%	95-102%	88-95%	95-104%
EDTA plasma(n=5)	93-105%	87-103%	81-93%	88-104%
Heparin plasma(n=5)	93-103%	81-96%	86-93%	93-102%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
40.00	1.956	1.867
20.00	1.593	1.504
10.00	1.106	1.017
5.00	0.847	0.758
2.50	0.539	0.450
1.25	0.354	0.265
0.63	0.225	0.136
0.00	0.089	0.000

Sensitivity

0.236 ng/mL

Limit Of Detection

40 ng/mL

Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken DAO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken DAO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken DAO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken DAO in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Enzyme & Kinase;
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE152011161	ELK9423	48T	$320		Add to Cart
BHE152011161	ELK9423	96T	$458		Add to Cart

Chicken DAO(Diamine Oxidase) ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Chicken DAO(Diamine Oxidase) ELISA Kit

Rate it now..!!