Matrices listed below were spiked with certain level of Calprotectin and the recovery rates were calculated by comparing the measured value to the expected amount of Calprotectin in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-103
92
EDTA plasma(n=5)
90-104
94
heparin plasma(n=5)
85-102
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Calprotectin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Calprotectin ELISA Kit allows for the in vitro quantitative determination of Calprotectin concentrations in serum, plasma, tissue homogenates and other biological fluids.