Chicken adrenocorticotropic hormone (ACTH) ELISA kit

Mfr.No.

CSB-E14373C

Sensitivity

0.125 pg/mL

Detection Range

0.25 pg/mL-50 pg/mL

Reactivity

Chicken

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

adrenocorticotropic hormone (ACTH)

Recovery

The recovery of bovine FN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	88-105
EDTA plasma (n=4)	97	92-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine FN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	92
	Range %	85-102
1:10	Average %	97
	Range %	91-110
1:20	Average %	89
	Range %	83-96
1:40	Average %	95
	Range %	84-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Gallus gallus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	ACTH

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Concentrations of the adrenocorticotropic hormone, corticosterone and sex steroid hormones and the expression of the androgen receptor in the pituitary and adrenal glands of male turkeys (Meleagris gallopavo) during growth and development. Kiezun J et al,Gen Comp Endocrinol,2015 Mar 14.pii: S0016-6480(15)00072-6. Concentrations of the adrenocorticotropic hormone, corticosterone and sex steroid hormones and the expression of the androgen receptor in the pituitary and adrenal glands of male turkeys (Meleagris gallopavo) during growth and development Kiezun J.et al,Gen Comp Endocrinol.,2015 217: 62-70. Effect of short-and long-term feed restriction on ghrelin concentrations in turkeys F R Vizcarra.et al,Poultry Science,Feb 28

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