Chicken adrenocorticotropic hormone (ACTH) ELISA kit

Mfr.No.

CSB-E14373C

Alternative Names

adrenocorticotropic hormone (ACTH)

Reactivity

Chicken

Detection Range

0.25 pg/mL-50 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of bovine FN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	88-105
EDTA plasma (n=4)	97	92-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine FN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	92
	Range %	85-102
1:10	Average %	97
	Range %	91-110
1:20	Average %	89
	Range %	83-96
1:40	Average %	95
	Range %	84-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

µg/ml	OD1	OD2	Average	Corrected
100	1.839	1.803	1.821	1.677
33	1.657	1.649	1.653	1.509
11	1.451	1.435	1.443	1.299
3.7	0.958	0.992	0.975	0.831
1.2	0.643	0.667	0.655	0.511
0.4	0.372	0.354	0.363	0.219
0.13	0.251	0.243	0.247	0.103
0	0.145	0.142	0.144	?

Species

Gallus gallus

Sensitivity

0.125 pg/mL

Limit Of Detection

450 nm

Antigen	Adrenocorticotropic Hormone (ACTH)

Applications

ELISA

Research Area

ACTH

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Concentrations of the adrenocorticotropic hormone, corticosterone and sex steroid hormones and the expression of the androgen receptor in the pituitary and adrenal glands of male turkeys (Meleagris gallopavo) during growth and development. Kiezun J et al,Gen Comp Endocrinol,2015 Mar 14.pii: S0016-6480(15)00072-6. Concentrations of the adrenocorticotropic hormone, corticosterone and sex steroid hormones and the expression of the androgen receptor in the pituitary and adrenal glands of male turkeys (Meleagris gallopavo) during growth and development Kiezun J.et al,Gen Comp Endocrinol.,2015 217: 62-70. Effect of short-and long-term feed restriction on ghrelin concentrations in turkeys F R Vizcarra.et al,Poultry Science,Feb 28

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SKU	Supplier No.	Size	Your price
BHE10500402	CSB-E14373C	96T	$790	Add to Cart
BHE10500402	CSB-E14373C	5x96T	$3792	Add to Cart
BHE10500402	CSB-E14373C	10x96T	$7505	Add to Cart

Chicken adrenocorticotropic hormone (ACTH) ELISA kit

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