General CBG(Cinobufagin) ELISA Kit

Mfr.No.

ELK8271

Sensitivity

91.3 ng/mL

Detection Range

312.5-20000 ng/mL

Reactivity

General

Sample Type

Other biological fluids

Assay Duration

Assay Type

Competitive Inhibition

Alternative Names

CBG

Recovery

Matrices listed below were spiked with certain level of recombinant CBG and the recovery rates were calculated by comparing the measured value to the expected amount of CBG in samples.

Matrix	Recovery range	Average
serum(n=5)	86-99%	92%
EDTA plasma(n=5)	89-103%	96%
Heparin plasma(n=5)	78-96%	88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CBG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	88-98%	87-103%	86-92%	89-97%
EDTA plasma(n=5)	85-94%	87-101%	86-97%	88-95%
Heparin plasma(n=5)	85-94%	78-96%	79-93%	82-90%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
20000.00	0.175
10000.00	0.386
5000.00	0.655
2500.00	0.895
1250.00	1.257
625.00	1.596
312.50	1.624
0.00	2.197

Limit Of Detection

20000 ng/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with CBG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CBG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CBG in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

Antigen	CBG

Applications	ELISA
Research Area	Endocrinology;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15200321	ELK8271	48T	$265		Add to Cart
BHE15200321	ELK8271	96T	$376		Add to Cart

CBG(Cinobufagin) ELISA Kit

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