Cattle sRAGE(Soluble receptor for advanced glycation endproducts) ELISA Kit

Mfr.No.

ELK9694

Alternative Names

sRAGE, sRAGE

Reactivity

Cattle

Detection Range

12.5-800 pg/mL, 12.5-800 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Other biological fluids, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant sRAGE and the recovery rates were calculated by comparing the measured value to the expected amount of sRAGE in samples.

Matrix	Recovery range	Average
serum(n=5)	96-107%	102%
EDTA plasma(n=5)	80-92%	86%
Heparin plasma(n=5)	88-103%	95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sRAGE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	95-106%	83-98%	93-105%	95-104%
EDTA plasma(n=5)	97-106%	85-98%	97-106%	86-92%
Heparin plasma(n=5)	91-103%	89-97%	85-94%	97-106%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
800.00	1.941	1.856
400.00	1.638	1.553
200.00	1.188	1.103
100.00	0.921	0.836
50.00	0.547	0.462
25.00	0.379	0.294
12.50	0.202	0.117
0.00	0.085	0.000

Sensitivity

3.93 pg/mL

Limit Of Detection

800 pg/mL

Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle sRAGE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle sRAGE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle sRAGE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle sRAGE in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Metabolic pathway;Tumor immunity;Endocrinology;Cardiovascular biology;Neuro science;
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE152011429	ELK9694	48T	$320		Add to Cart
BHE152011429	ELK9694	96T	$458		Add to Cart

Cattle sRAGE(Soluble receptor for advanced glycation endproducts) ELISA Kit

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