Cattle SPD(Surfactant Associated Protein D) ELISA Kit

Mfr.No.

ELK9743

Alternative Names

SFTPD; COLEC7; PSP-D; SFTP4; SP-D; Pulmonary Surfactant Protein D; Collectin-7; Lung surfactant protein D

Reactivity

Cattle

Detection Range

0.63-40 ng/mL

Sample Type

Tissue homogenates, Cell lysates, Lung lavage fluid, Other biological fluids, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant SPD and the recovery rates were calculated by comparing the measured value to the expected amount of SPD in samples.

Matrix	Recovery range	Average
serum(n=5)	87-99%	93%
EDTA plasma(n=5)	80-95%	87%
Heparin plasma(n=5)	91-105%	98%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SPD and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	91-99%	83-89%	88-104%	83-92%
EDTA plasma(n=5)	96-102%	81-93%	79-91%	89-101%
Heparin plasma(n=5)	85-96%	93-102%	89-97%	85-94%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
40.00	2.266	2.179
20.00	1.572	1.485
10.00	1.136	1.049
5.00	0.896	0.809
2.50	0.557	0.470
1.25	0.326	0.239
0.63	0.212	0.125
0.00	0.087	0.000

Species

Cattle

Sensitivity

0.237 ng/mL

Limit Of Detection

40 ng/mL

UniProt ID	P35246
Antigen	SPD
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle SPD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle SPD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle SPD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle SPD in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Infection immunity;Pulmonology;
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE152011486	ELK9743	48T	$265		Add to Cart
BHE152011486	ELK9743	96T	$376		Add to Cart

Cattle SPD(Surfactant Associated Protein D) ELISA Kit

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