Cattle RANTES(Regulated On Activation In Normal T-Cell Expressed And Secreted) ELISA Kit

Mfr.No.

ELK9153

Alternative Names

CCL5; SCYA5; SISd; EoCP; Chemokine C-C-Motif Ligand 5; Eosinophil Chemotactic Cytokine; T-cell Specific Protein p288; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 5

Reactivity

Cattle

Detection Range

3.13-200 ng/mL

Sample Type

Other biological fluids, Serum, Plasma

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant RANTES and the recovery rates were calculated by comparing the measured value to the expected amount of RANTES in samples.

Matrix	Recovery range	Average
serum(n=5)	89-107%	98%
EDTA plasma(n=5)	94-107%	100%
Heparin plasma(n=5)	81-93%	87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RANTES and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	86-93%	81-94%	89-98%	90-99%
EDTA plasma(n=5)	95-106%	87-101%	86-93%	93-102%
Heparin plasma(n=5)	87-98%	83-97%	91-98%	83-101%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
200.00	2.135	2.063
100.00	1.628	1.556
50.00	1.194	1.122
25.00	0.828	0.756
12.50	0.496	0.424
6.25	0.357	0.285
3.13	0.199	0.127
0.00	0.072	0.000

Sensitivity

1.31 ng/mL

Limit Of Detection

200 ng/mL

Antigen	RANTES
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle RANTES. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle RANTES. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle RANTES, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle RANTES in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Cytokine;Tumor immunity;Infection immunity;Immune molecule;
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15207483	ELK9153	48T	$412		Add to Cart
BHE15207483	ELK9153	96T	$588		Add to Cart

Cattle RANTES(Regulated On Activation In Normal T-Cell Expressed And Secreted) ELISA Kit

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