Canine Vasoactive Intestinal Peptide

Mfr.No.

E0290Ca

Description

This sandwich kit is for the accurate quantitative detection of Canine Vasoactive Intestinal Peptide (also known as VIP) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Vasoactive intestinal Peptide;Vasoactive intestinal polypeptide;VIP

Reactivity

Canine

Detection Range

2-700ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

3327

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

1.53ng/L

Background

VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder.

Gene ID	484038
UniProt ID	P63289
Antigen	VIP

Research Area

Neuroscience

Protocol

These standard curves of E0290Ca are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
400ng/L	2.104	2.128	2.079
	2.152
200ng/L	1.57	1.526	1.476
	1.482
100ng/L	1.128	1.129	1.079
	1.13
50ng/L	0.643	0.663	0.614
	0.684
25ng/L	0.448	0.426	0.377
	0.405
0ng/L	0.065	0.05	0
	0.034

Precision

We measured random samples of E0290Ca within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	87.77	6.84	7.79
Intra-Assay	2	18	287.43	17.89	6.22
Intra-Assay	3	18	24.51	1.91	7.8

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100801	E0290Ca	48T	$350		Add to Cart
BHE12100801	E0290Ca	96T	$475		Add to Cart

Canine Vasoactive Intestinal Peptide - VIP ELISA Kit

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