Canine Vascular Cell Adhesion Molecule 1

Mfr.No.

E0020Ca

Sensitivity

1.24ng/ml

Detection Range

2-600ng/ml

Reactivity

Canine

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Vascuolar cell adhesion molecule 1 (also known as VCAM-1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CD antigen CD106;Vascular cell adhesion protein 1;VCAM 1;V-CAM 1;VCAM1;VCAM-1

Molecular Weight

81412

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0020Ca within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	150.4	7.65	5.1
Intra-Assay	2	18	227.9	5.42	2.4
Intra-Assay	3	18	61.8	2.93	4.7

Background

Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation (By similarity).

Protocol

These standard curves of E0020Ca are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
320ng/ml	2.387	2.315	2.295
	2.243
160ng/ml	1.188	1.189	1.17
	1.191
80ng/ml	0.678	0.644	0.624
	0.609
40ng/ml	0.363	0.354	0.334
	0.345
20ng/ml	0.164	0.167	0.147
	0.17
0ng/ml	0.019	0.02	0
	0.021

Gene ID	403982
UniProt ID	Q28260

Research Area	Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100573	E0020Ca	96T	$475		Add to Cart
BHE12100573	E0020Ca	48T	$350		Add to Cart

Canine Vascular Cell Adhesion Molecule 1 - VCAM-1 ELISA Kit

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