Canine Tryptase - TPS ELISA Kit

Mfr.No.

E0247Ca

Sensitivity

0.023ng/ml

Detection Range

0.05-15ng/ml

Reactivity

Canine

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Canine Tryptase (also known as TPS) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

TPS;Tryptase

Molecular Weight

30088

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0247Ca within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.2	0.13	4.1
Intra-Assay	2	18	5.5	0.16	2.9
Intra-Assay	3	18	2.2	0.09	4.2

Background

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type.

Protocol

These standard curves of E0247Ca are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/ml	1.865	1.841	1.806
	1.816
4ng/ml	1.087	1.054	1.02
	1.021
2ng/ml	0.469	0.467	0.433
	0.466
1ng/ml	0.174	0.24	0.206
	0.307
0.5ng/ml	0.085	0.126	0.091
	0.167
0ng/ml	0.034	0.035	0
	0.036

Gene ID	100049001
UniProt ID	P15944

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100768	E0247Ca	96T	$475		Add to Cart
BHE12100768	E0247Ca	48T	$350		Add to Cart

Canine Tryptase - TPS ELISA Kit

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