Matrices listed below were spiked with certain level of TNF-alpha and the recovery rates were calculated by comparing the measured value to the expected amount of TNF-alpha in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-100
92
EDTA plasma(n=5)
90-105
96
heparin plasma(n=5)
85-105
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNF-alpha and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
TNF-alpha ELISA Kit allows for the in vitro quantitative determination of TNF-alpha concentrations in serum, plasma, tissue homogenates and other biological fluids.