Matrices listed below were spiked with certain level of TFF3 and the recovery rates were calculated by comparing the measured value to the expected amount of TFF3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-104
92
EDTA plasma(n=5)
86-100
91
heparin plasma(n=5)
87-105
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TFF3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
82-98%
80-105%
80-97%
EDTA plasma(n=5)
90-100%
92-101%
91-101%
heparin plasma(n=5)
90-98%
90-95%
90-100%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
Q863B4
Applications
ELISA
Applications Notes
TFF3 ELISA kit allows for the in vitro quantitative determination of TFF3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Canine TFF3 (Trefoil factor 3) ELISA kit