Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of RETN and the recovery rates were calculated by comparing the measured value to the expected amount of RETN in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-96
91
EDTA plasma(n=5)
88-103
96
heparin plasma(n=5)
85-103
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RETN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-104%
93-104%
86-102%
EDTA plasma(n=5)
87-96%
87-97%
83-100%
heparin plasma(n=5)
82-98%
83-100%
84-97%
Sensitivity
< 1.875pg/ml
Notes
For Research Use Only
UniProt ID
A0A8C0PKD7
Applications
ELISA
Applications Notes
RETN ELISA Kit allows for the in vitro quantitative determination of RETN concentrations in serum, plasma, tissue homogenates and other biological fluids.