Canine Relaxin(RLN) ELISA Kit

Mfr.No.

CSB-E14982c

Sensitivity

0.78 pg/mL

Detection Range

3.12 pg/mL-200 pg/mL

Reactivity

Canis familiaris, Dog

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Relaxin(RLN)

Recovery

The recovery of canine CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	95-107
EDTA plasma (n=4)	89	85-93

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of canine CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	84
	Range %	80-88
1:200	Average %	98
	Range %	94-102
1:400	Average %	90
	Range %	86-94
1:800	Average %	92
	Range %	88-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Canis lupus familiaris

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	Q9TRM8

Applications	ELISA
Research Area	RLN

Storage	Store at 2-8°C. Please refer to protocol.

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