Canine Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

Mfr.No.

CSB-E14930c

Sensitivity

7.5 uIU/mL

Detection Range

7.5 uIU/mL-3000 uIU/mL

Reactivity

Canis familiaris, Dog

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Prolactin/Luteotropic Hormone(PRL/LTH)

Recovery

The recovery of human COLEC12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	90	85-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human COLEC12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-88
1:2	Average %	85
	Range %	82-89
1:4	Average %	91
	Range %	86-95
1:8	Average %	104
	Range %	101-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Canis lupus familiaris

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	PRL, LTH

Storage	Store at 2-8°C. Please refer to protocol.

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