Canine Orexin - HCRT ELISA Kit

Mfr.No.

E0365Ca

Sensitivity

0.27ng/L

Detection Range

0.5-200ng/L

Reactivity

Canine

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

HCRT;Hcrt1;Hcrt2;Hypocretin;Hypocretin-1;Hypocretin-2;Orexin;Orexin-A;Orexin-B;

Molecular Weight

13328

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0365Ca within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	18.2	1.1	6
Intra-Assay	2	18	33.1	1.36	4.1
Intra-Assay	3	18	86.7	2.56	3

Background

Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity.

Protocol

These standard curves of E0365Ca are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
120ng/L	2.293	2.283	2.247
	2.273
60ng/L	1.372	1.304	1.268
	1.236
30ng/L	0.624	0.612	0.576
	0.6
15ng/L	0.404	0.389	0.353
	0.373
7.5ng/L	0.179	0.191	0.155
	0.202
0ng/L	0.053	0.036	0
	0.019

Gene ID	607641
UniProt ID	Q9GLF6

Research Area	Metabolism

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100873	E0365Ca	96T	$475		Add to Cart
BHE12100873	E0365Ca	48T	$350		Add to Cart

Canine Orexin - HCRT ELISA Kit

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