Canine Occludin - ELISA Kit

Mfr.No.

E0013Ca

Sensitivity

2.38ng/L

Detection Range

5-1500ng/L

Reactivity

Canine

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Occludin 1 (also known as Occludin 1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Occludin;OCLN

Molecular Weight

59275

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0013Ca within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	451.7	14.37	3.2
Intra-Assay	2	18	305.6	15.7	5.1
Intra-Assay	3	18	154.8	6.42	4.1

Background

May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. Interacts with ZO-1.

Protocol

These standard curves of E0013Ca are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/L	2.117	2.189	2.146
	2.262
400ng/L	1.432	1.412	1.369
	1.392
200ng/L	0.602	0.696	0.652
	0.789
100ng/L	0.344	0.382	0.339
	0.42
50ng/L	0.138	0.169	0.126
	0.2
0ng/L	0.041	0.043	0
	0.046

Gene ID	403844
UniProt ID	Q28269

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100567	E0013Ca	96T	$475		Add to Cart
BHE12100567	E0013Ca	48T	$350		Add to Cart

Canine Occludin - ELISA Kit

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