Matrices listed below were spiked with certain level of NGF/NGFbeta and the recovery rates were calculated by comparing the measured value to the expected amount of NGF/NGFbeta in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-104
95
EDTA plasma(n=5)
85-96
92
heparin plasma(n=5)
85-104
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NGF/NGFbeta and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-99%
85-101%
88-104%
EDTA plasma(n=5)
88-101%
82-98%
88-101%
heparin plasma(n=5)
83-97%
81-100%
82-95%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
A0A8C0MBJ0
Applications
ELISA
Applications Notes
NGF/NGFbeta ELISA Kit allows for the in vitro quantitative determination of NGF/NGFbeta concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Canine NGF/NGFbeta(Beta-nerve growth factor) ELISA Kit