Canine Luteinizing Hormone,LH ELISA Kit

Mfr.No.

CSB-E06790c

Alternative Names

Luteinizing Hormone,LH

Reactivity

Canis familiaris, Dog

Detection Range

0.75 mIU/mL-60 mIU/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of horse PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-105
EDTA plasma (n=4)	97	88-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of horse PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-101
1:2	Average %	95
	Range %	85-100
1:4	Average %	91
	Range %	89-99
1:8	Average %	93
	Range %	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Canis lupus familiaris

Sensitivity

0.94 mIU/mL

Limit Of Detection

450 nm

UniProt ID	P18842
Antigen	Luteinizing Hormone,LH

Applications

ELISA

Research Area

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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