Canine Interleukin 3 - IL-3 ELISA Kit

Mfr.No.

E0137Ca

Sensitivity

0.49ng/L

Detection Range

1-300ng/L

Reactivity

Canine

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Interleukin 3 (also known as IL-3) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

Hematopoietic growth factor;IL 3;IL3;IL-3;Interleukin-3;Mast cell growth factor;MCGF;Multipotential colony-stimulating factor;P-cell-stimulating factor;

Molecular Weight

16499

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E0137Ca within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	149.2	4.67	3.1
Intra-Assay	2	18	84	4.23	5
Intra-Assay	3	18	38.8	1.85	4.8

Background

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.

Protocol

These standard curves of E0137Ca are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
160ng/L	1.874	1.869	1.825
	1.864
80ng/L	0.988	0.949	0.905
	0.91
40ng/L	0.438	0.526	0.482
	0.614
20ng/L	0.19	0.21	0.166
	0.23
10ng/L	0.124	0.13	0.086
	0.136
0ng/L	0.026	0.044	0
	0.062

Gene ID	481497
UniProt ID	Q9BDX4

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100678	E0137Ca	96T	$475		Add to Cart
BHE12100678	E0137Ca	48T	$350		Add to Cart

Canine Interleukin 3 - IL-3 ELISA Kit

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