Canine Interferon Gamma - IFN-G ELISA Kit

Mfr.No.

E0011Ca

Sensitivity

2.58ng/L

Detection Range

5-1500ng/L

Reactivity

Canine

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Interferon γ (also known as IFN-γ) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

IFN G;IFN gamma;IFNG;IFN-gamma;Interferon gamma

Molecular Weight

19395

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0011Ca within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	600.3	29.8	5
Intra-Assay	2	18	223.7	13.39	6
Intra-Assay	3	18	95.4	6.95	7.3

Background

Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Protocol

These standard curves of E0011Ca are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
960ng/L	2.245	2.147	2.099
	2.048
480ng/L	1.61	1.608	1.561
	1.607
240ng/L	1.026	1.008	0.96
	0.989
120ng/L	0.753	0.702	0.655
	0.652
60ng/L	0.476	0.437	0.389
	0.397
0ng/L	0.053	0.048	0
	0.042

Gene ID	403801
UniProt ID	P42161

Research Area	Immunology; Stem Cells; Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100565	E0011Ca	96T	$475		Add to Cart
BHE12100565	E0011Ca	48T	$350		Add to Cart

Canine Interferon Gamma - IFN-G ELISA Kit

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